The BLAST database used hg38.fa.gz (which I believe to be patch 12) from here. That was unzipped and processed with makeblastdb . Primer-BLAST Results ... ... <看更多>
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The BLAST database used hg38.fa.gz (which I believe to be patch 12) from here. That was unzipped and processed with makeblastdb . Primer-BLAST Results ... ... <看更多>
In addition to running primer3 to suggest primers, Primer-BLAST checks against the possibility of unwanted. PCR products by comparing potential primers ... ... <看更多>