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在crispr-cas9 knockout這個產品中，有1篇Facebook貼文，粉絲數超過4萬的網紅北歐心科學 NordicHearts，也在其Facebook貼文中提到， 另一位分子生物學朋友的看法。 事態極嚴重！中共國又一違天力作——基因改造人誕生！ 今次事出突然，廿四小時內，《自然》也有相關報道。 筆者先從科學角度解釋一下。事件為有科學家利用 CRISPR / Cas9 技術，把人類胚胎的 CCR5 基因敲除，並使她們（雙生女）順利出生。雖然尚未證實真...

crispr-cas9 knockout 在 北歐心科學 NordicHearts Facebook 的精選貼文

北歐心科學 NordicHearts By 北歐心科學 NordicHearts

2018-11-27 01:41:56 有 104 人按讚
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另一位分子生物學朋友的看法。

事態極嚴重！中共國又一違天力作——基因改造人誕生！

今次事出突然，廿四小時內，《自然》也有相關報道。

筆者先從科學角度解釋一下。事件為有科學家利用 CRISPR / Cas9 技術，把人類胚胎的 CCR5 基因敲除，並使她們（雙生女）順利出生。雖然尚未證實真偽，但兩人或成為有紀錄以來首兩個基因改造人。

CCR5 是人類 T 免疫細胞的受體（receptor），也是愛滋病毒入侵 T 細胞的必經之路。敲除（knockout）CCR5，兩個女嬰理論上是終生對愛滋病免疫的。

然後，要討論醫藥安全道德問題。雖則敲除 CCR5 是治療愛滋病毒的方法，但是現階段尚未完全通過測試。而且，這個基因的研究是在免疫細胞中敲除，並非全身所有細胞。科學界尚未了解 CCR5 在其他細胞上有否作用。另一方面，CRISPR / Cas9 會脫靶（off target），即是說女嬰的基因組或出現未知的突變，較易引發癌症。換句話說，這次醫療事件的安全是沒有評估的，沒有臨床實驗、安全測試、醫療監管。

下一個，是科學道德問題——人類應否修改下一代的遺傳基因。科學界對於一些明顯的遺傳病，是持較開放態度的。但仍然十分緊慎。道德觀念基於我們沒有權利去操控別人（包括親生子女）。而改變遺傳基因，卻是生物學上最強的「操控」。另一個道德考慮，是要避免人類陷入無止境的改造競賽。改遺傳病是把病人變為一般人；但改傳染病的抵抗力是把一般人「改良」成為更優越者。會衍生出改膚色、體格、智商，等等一系列的問題。

而今次的科學家很「聰明」，選擇挑戰道德臨界線。父親是愛滋病患者，女嬰易被傳染。改了胚胎基因，就把女嬰的愛滋病「根除」。一次過衝擊兩個道德觀念。「女嬰患上不治之症很無辜啊！父親時常害怕傳染給女兒很悲慘啊！」這樣一想，你的同情心和理性就被收割了。

最後，是政治問題。筆者一向少在此專頁藉科學談政治，但今次破例。在筆者眼中，政治就是陰謀論。該科研團隊在此時此刻發表違反科學界主流道德觀的實驗，明顯對中共不利。美國準備對中共實施高科技禁運，用的理由是違反人權道德——中共的人民監控計劃。而是次事件，中共的生物科技也是違反人權道德。這有意無意給了美國彈藥，為下一個高科技禁運作部署。更有趣的，在於《自然》的相關報道，特意提到香港。而香港又正正在高科技禁運的清單內，罪名是——香港協助把相關科技轉運到中國。一切是巧合嗎？

這次，筆者很希望該實驗是造假的。

https://www.nature.com/articles/d41586-018-07545-0…

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crispr-cas9 knockout 在 CRISPR Guide - Addgene 的相關結果

You can use CRISPR to generate knockout cells or animals by co-expressing an endonuclease like Cas9 or Cas12a (also known as Cpf1) and a gRNA specific to ... ... <看更多>

crispr-cas9 knockout 在 CRISPR/Cas9 Gene Knockout Kits - Genome Editing Tools 的相關結果

CRISPR /Cas9 is a simple and efficient genome editing tool. Although gene knockout cell lines can be generated by gRNAs without donor vector, the screening ... ... <看更多>

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